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Under these different culture conditions, phenotype, cell metabolomic profiles, response to maturation stimuli, cytokines production, allogenic T cell stimulatory capacity, as well as priming of antigen-specific CD8 + T cells and activation of autologous natural killer (NK) cells were analyzed. Here we sought to compare the ability of three clinical grade serum-free media, DendriMACS, AIM-V, and X-VIVO 15, alongside with fetal bovine serum-supplemented Roswell Park Memorial Institute Medium (RPMI), to support the differentiation of monocyte-derived DCs (Mo-DCs). Translation to the clinic has been hindered in part by the lack of standard operation procedures for vaccines production, namely the definition of optimal culture conditions during ex-vivo DC differentiation.

8Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT), University of Coimbra, Coimbra, Portugalĭendritic cell (DC)-based antitumor vaccines have proven to be a safe approach, but often fail to generate robust results between trials.

6Center for Innovation in Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, Portugal.

5Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Coimbra, Portugal.

4Department of Medical Sciences and Institute of Biomedicine (iBiMED), University of Aveiro, Aveiro, Portugal.

3CICECO, Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal.

2Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra, Portugal.

1Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.Tavares 1,2, Daniel Alexandre Ferreira 5, Célia Gomes 5,6, João Serra 7, Amílcar Falcão 1,8, Maria Teresa Cruz 1,2, Mylène A.

João Calmeiro 1,2, Luís Mendes 3, Iola F.